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bronchial tracheal epithelial cells  (ATCC)


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    Structured Review

    ATCC bronchial tracheal epithelial cells
    Bronchial Tracheal Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tracheal+epithelial+cells/Primary+Bronchial%2FTracheal+Epithelial+Cells%3B+Asthma/us12582619-622-18-21
    Average 99 stars, based on 10 article reviews
    bronchial tracheal epithelial cells - by Bioz Stars, 2026-11
    99/100 stars

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    Staining:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin, TPCK Treated Thermo Fisher Cat #PI20233 Trypsin-EDTA (0.25%) phenol red Thermo Fisher Cat #25200056 Vancomycin hydrochloride Millipore Sigma Cat #94747-5G Critical commercial assays ArCTM Amine Reactive Compensation Bead Kit Thermo Fisher Cat #A10346 BD Cytofix/Cytoperm BD Biosciences Cat #554722 BD Perm/Wash BD Biosciences Cat #554723 Brilliant Stain Buffer BD Bioscience Cat #566349 DNeasy 96 PowerSoil Pro Kit QIAGEN Cat #47017 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientifc (eBiocience) Cat #00-5523-00 Human IFN-beta Quantikine ELISA Kit Bio-Techne Cat #DIFNB0 IFN-beta Quantikine ELISA Kit Bio-Techne Cat #MIFNB0 iScriptTM cDNA Synthesis Kit Bio-Rad Cat #1708891 LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) BioLegend Cat #740622 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit Thermo Fischer Cat #L34962 OneComp eBeadsTM Compensation Beads Thermo Fisher Cat #01-1111-42 QuantiNova SYBR Green PCR Kit QIAGEN Cat #208054 RNeasy Micro kit QIAGEN Cat #74004 RNeasy Mini kit QIAGEN Cat #73404 Quick-DNA Fecal/Soil Microbe Miniprep Kit Zymo Research Cat #D6010 Deposited data Raw RNA-seq data This paper GEO: GSE299495 Code and associated analysis This paper Figshare: https://doi.org/10.6084/ m9.figshare.31955649 Raw 16S rRNA-seq data This paper NCBI SRA: PRJNA1450632 Experimental models: Cell lines MDCK (NBL-2) cells ATCC Cat # CCL-34 Primary Bronchial/Tracheal epithelial cells; normal human ATCC Cat #PCS-300-010 Vero cells ATCC Cat #CCL-81 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Mouse Strain: Jax 000664; RRID: IMSR_JAX:000664 Mouse: B6.129S2-Ighmtm1Cgn/J Jackson Laboratory Mouse Strain: 002288; RRID: IMSR_JAX:002288 Mouse: C57BL/6N Germ free Taconic Bioscience Mouse Strain: C57BL/6NTac; RRID: IMSR_TAC:B6 Oligonucleotides KiCqStartTM Primer: Actb Forward: CATTGCTGACAGGATGCAGAAGG Millipore Sigma N/A KiCqStartTM Primer: Actb Reverse: TGCTGGAAGGTGGACAGTGAGG Millipore Sigma N/A KiCqStartTM Primer: Irf9 Forward: CTACTTCTGTAGAGATTTGGC Millipore Sigma N/A KiCqStartTM Primer: Irf9 Reverse: GATGAGATTCTCTTGGCTATG Millipore Sigma N/A (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e9, June 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStartTM Primer: Isg15 Forward: ATGGAGGACTTTTGGGATAG Millipore Sigma N/A KiCqStartTM Primer: Isg15 Reverse: AGAGGCAGAGCTTTTTATTG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Forward: CTACGCAAATCTGATCAAGG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Reverse: CACTTGTCTCTCACATACTG Millipore Sigma N/A KiCqStartTM Primer: Stat1 Forward: TTTGACAGTATGATGAGCAC Millipore Sigma N/A KiCqStartTM Primer: Stat1 Reverse: AGCAAATGTGATGCTCTTTC Millipore Sigma N/A Lactobacillus johnsonii 16S–23S region.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin, TPCK Treated Thermo Fisher Cat #PI20233 Trypsin-EDTA (0.25%) phenol red Thermo Fisher Cat #25200056 Vancomycin hydrochloride Millipore Sigma Cat #94747-5G Critical commercial assays ArCTM Amine Reactive Compensation Bead Kit Thermo Fisher Cat #A10346 BD Cytofix/Cytoperm BD Biosciences Cat #554722 BD Perm/Wash BD Biosciences Cat #554723 Brilliant Stain Buffer BD Bioscience Cat #566349 DNeasy 96 PowerSoil Pro Kit QIAGEN Cat #47017 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientifc (eBiocience) Cat #00-5523-00 Human IFN-beta Quantikine ELISA Kit Bio-Techne Cat #DIFNB0 IFN-beta Quantikine ELISA Kit Bio-Techne Cat #MIFNB0 iScriptTM cDNA Synthesis Kit Bio-Rad Cat #1708891 LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) BioLegend Cat #740622 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit Thermo Fischer Cat #L34962 OneComp eBeadsTM Compensation Beads Thermo Fisher Cat #01-1111-42 QuantiNova SYBR Green PCR Kit QIAGEN Cat #208054 RNeasy Micro kit QIAGEN Cat #74004 RNeasy Mini kit QIAGEN Cat #73404 Quick-DNA Fecal/Soil Microbe Miniprep Kit Zymo Research Cat #D6010 Deposited data Raw RNA-seq data This paper GEO: GSE299495 Code and associated analysis This paper Figshare: https://doi.org/10.6084/ m9.figshare.31955649 Raw 16S rRNA-seq data This paper NCBI SRA: PRJNA1450632 Experimental models: Cell lines MDCK (NBL-2) cells ATCC Cat # CCL-34 Primary Bronchial/Tracheal epithelial cells; normal human ATCC Cat #PCS-300-010 Vero cells ATCC Cat #CCL-81 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Mouse Strain: Jax 000664; RRID: IMSR_JAX:000664 Mouse: B6.129S2-Ighmtm1Cgn/J Jackson Laboratory Mouse Strain: 002288; RRID: IMSR_JAX:002288 Mouse: C57BL/6N Germ free Taconic Bioscience Mouse Strain: C57BL/6NTac; RRID: IMSR_TAC:B6 Oligonucleotides KiCqStartTM Primer: Actb Forward: CATTGCTGACAGGATGCAGAAGG Millipore Sigma N/A KiCqStartTM Primer: Actb Reverse: TGCTGGAAGGTGGACAGTGAGG Millipore Sigma N/A KiCqStartTM Primer: Irf9 Forward: CTACTTCTGTAGAGATTTGGC Millipore Sigma N/A KiCqStartTM Primer: Irf9 Reverse: GATGAGATTCTCTTGGCTATG Millipore Sigma N/A (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e9, June 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStartTM Primer: Isg15 Forward: ATGGAGGACTTTTGGGATAG Millipore Sigma N/A KiCqStartTM Primer: Isg15 Reverse: AGAGGCAGAGCTTTTTATTG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Forward: CTACGCAAATCTGATCAAGG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Reverse: CACTTGTCTCTCACATACTG Millipore Sigma N/A KiCqStartTM Primer: Stat1 Forward: TTTGACAGTATGATGAGCAC Millipore Sigma N/A KiCqStartTM Primer: Stat1 Reverse: AGCAAATGTGATGCTCTTTC Millipore Sigma N/A Lactobacillus johnsonii 16S–23S region.

    cDNA Synthesis:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin, TPCK Treated Thermo Fisher Cat #PI20233 Trypsin-EDTA (0.25%) phenol red Thermo Fisher Cat #25200056 Vancomycin hydrochloride Millipore Sigma Cat #94747-5G Critical commercial assays ArCTM Amine Reactive Compensation Bead Kit Thermo Fisher Cat #A10346 BD Cytofix/Cytoperm BD Biosciences Cat #554722 BD Perm/Wash BD Biosciences Cat #554723 Brilliant Stain Buffer BD Bioscience Cat #566349 DNeasy 96 PowerSoil Pro Kit QIAGEN Cat #47017 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientifc (eBiocience) Cat #00-5523-00 Human IFN-beta Quantikine ELISA Kit Bio-Techne Cat #DIFNB0 IFN-beta Quantikine ELISA Kit Bio-Techne Cat #MIFNB0 iScriptTM cDNA Synthesis Kit Bio-Rad Cat #1708891 LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) BioLegend Cat #740622 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit Thermo Fischer Cat #L34962 OneComp eBeadsTM Compensation Beads Thermo Fisher Cat #01-1111-42 QuantiNova SYBR Green PCR Kit QIAGEN Cat #208054 RNeasy Micro kit QIAGEN Cat #74004 RNeasy Mini kit QIAGEN Cat #73404 Quick-DNA Fecal/Soil Microbe Miniprep Kit Zymo Research Cat #D6010 Deposited data Raw RNA-seq data This paper GEO: GSE299495 Code and associated analysis This paper Figshare: https://doi.org/10.6084/ m9.figshare.31955649 Raw 16S rRNA-seq data This paper NCBI SRA: PRJNA1450632 Experimental models: Cell lines MDCK (NBL-2) cells ATCC Cat # CCL-34 Primary Bronchial/Tracheal epithelial cells; normal human ATCC Cat #PCS-300-010 Vero cells ATCC Cat #CCL-81 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Mouse Strain: Jax 000664; RRID: IMSR_JAX:000664 Mouse: B6.129S2-Ighmtm1Cgn/J Jackson Laboratory Mouse Strain: 002288; RRID: IMSR_JAX:002288 Mouse: C57BL/6N Germ free Taconic Bioscience Mouse Strain: C57BL/6NTac; RRID: IMSR_TAC:B6 Oligonucleotides KiCqStartTM Primer: Actb Forward: CATTGCTGACAGGATGCAGAAGG Millipore Sigma N/A KiCqStartTM Primer: Actb Reverse: TGCTGGAAGGTGGACAGTGAGG Millipore Sigma N/A KiCqStartTM Primer: Irf9 Forward: CTACTTCTGTAGAGATTTGGC Millipore Sigma N/A KiCqStartTM Primer: Irf9 Reverse: GATGAGATTCTCTTGGCTATG Millipore Sigma N/A (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e9, June 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStartTM Primer: Isg15 Forward: ATGGAGGACTTTTGGGATAG Millipore Sigma N/A KiCqStartTM Primer: Isg15 Reverse: AGAGGCAGAGCTTTTTATTG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Forward: CTACGCAAATCTGATCAAGG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Reverse: CACTTGTCTCTCACATACTG Millipore Sigma N/A KiCqStartTM Primer: Stat1 Forward: TTTGACAGTATGATGAGCAC Millipore Sigma N/A KiCqStartTM Primer: Stat1 Reverse: AGCAAATGTGATGCTCTTTC Millipore Sigma N/A Lactobacillus johnsonii 16S–23S region.

    SYBR Green Assay:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin, TPCK Treated Thermo Fisher Cat #PI20233 Trypsin-EDTA (0.25%) phenol red Thermo Fisher Cat #25200056 Vancomycin hydrochloride Millipore Sigma Cat #94747-5G Critical commercial assays ArCTM Amine Reactive Compensation Bead Kit Thermo Fisher Cat #A10346 BD Cytofix/Cytoperm BD Biosciences Cat #554722 BD Perm/Wash BD Biosciences Cat #554723 Brilliant Stain Buffer BD Bioscience Cat #566349 DNeasy 96 PowerSoil Pro Kit QIAGEN Cat #47017 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientifc (eBiocience) Cat #00-5523-00 Human IFN-beta Quantikine ELISA Kit Bio-Techne Cat #DIFNB0 IFN-beta Quantikine ELISA Kit Bio-Techne Cat #MIFNB0 iScriptTM cDNA Synthesis Kit Bio-Rad Cat #1708891 LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) BioLegend Cat #740622 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit Thermo Fischer Cat #L34962 OneComp eBeadsTM Compensation Beads Thermo Fisher Cat #01-1111-42 QuantiNova SYBR Green PCR Kit QIAGEN Cat #208054 RNeasy Micro kit QIAGEN Cat #74004 RNeasy Mini kit QIAGEN Cat #73404 Quick-DNA Fecal/Soil Microbe Miniprep Kit Zymo Research Cat #D6010 Deposited data Raw RNA-seq data This paper GEO: GSE299495 Code and associated analysis This paper Figshare: https://doi.org/10.6084/ m9.figshare.31955649 Raw 16S rRNA-seq data This paper NCBI SRA: PRJNA1450632 Experimental models: Cell lines MDCK (NBL-2) cells ATCC Cat # CCL-34 Primary Bronchial/Tracheal epithelial cells; normal human ATCC Cat #PCS-300-010 Vero cells ATCC Cat #CCL-81 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Mouse Strain: Jax 000664; RRID: IMSR_JAX:000664 Mouse: B6.129S2-Ighmtm1Cgn/J Jackson Laboratory Mouse Strain: 002288; RRID: IMSR_JAX:002288 Mouse: C57BL/6N Germ free Taconic Bioscience Mouse Strain: C57BL/6NTac; RRID: IMSR_TAC:B6 Oligonucleotides KiCqStartTM Primer: Actb Forward: CATTGCTGACAGGATGCAGAAGG Millipore Sigma N/A KiCqStartTM Primer: Actb Reverse: TGCTGGAAGGTGGACAGTGAGG Millipore Sigma N/A KiCqStartTM Primer: Irf9 Forward: CTACTTCTGTAGAGATTTGGC Millipore Sigma N/A KiCqStartTM Primer: Irf9 Reverse: GATGAGATTCTCTTGGCTATG Millipore Sigma N/A (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e9, June 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStartTM Primer: Isg15 Forward: ATGGAGGACTTTTGGGATAG Millipore Sigma N/A KiCqStartTM Primer: Isg15 Reverse: AGAGGCAGAGCTTTTTATTG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Forward: CTACGCAAATCTGATCAAGG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Reverse: CACTTGTCTCTCACATACTG Millipore Sigma N/A KiCqStartTM Primer: Stat1 Forward: TTTGACAGTATGATGAGCAC Millipore Sigma N/A KiCqStartTM Primer: Stat1 Reverse: AGCAAATGTGATGCTCTTTC Millipore Sigma N/A Lactobacillus johnsonii 16S–23S region.

    Polymerase Chain Reaction:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin, TPCK Treated Thermo Fisher Cat #PI20233 Trypsin-EDTA (0.25%) phenol red Thermo Fisher Cat #25200056 Vancomycin hydrochloride Millipore Sigma Cat #94747-5G Critical commercial assays ArCTM Amine Reactive Compensation Bead Kit Thermo Fisher Cat #A10346 BD Cytofix/Cytoperm BD Biosciences Cat #554722 BD Perm/Wash BD Biosciences Cat #554723 Brilliant Stain Buffer BD Bioscience Cat #566349 DNeasy 96 PowerSoil Pro Kit QIAGEN Cat #47017 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientifc (eBiocience) Cat #00-5523-00 Human IFN-beta Quantikine ELISA Kit Bio-Techne Cat #DIFNB0 IFN-beta Quantikine ELISA Kit Bio-Techne Cat #MIFNB0 iScriptTM cDNA Synthesis Kit Bio-Rad Cat #1708891 LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) BioLegend Cat #740622 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit Thermo Fischer Cat #L34962 OneComp eBeadsTM Compensation Beads Thermo Fisher Cat #01-1111-42 QuantiNova SYBR Green PCR Kit QIAGEN Cat #208054 RNeasy Micro kit QIAGEN Cat #74004 RNeasy Mini kit QIAGEN Cat #73404 Quick-DNA Fecal/Soil Microbe Miniprep Kit Zymo Research Cat #D6010 Deposited data Raw RNA-seq data This paper GEO: GSE299495 Code and associated analysis This paper Figshare: https://doi.org/10.6084/ m9.figshare.31955649 Raw 16S rRNA-seq data This paper NCBI SRA: PRJNA1450632 Experimental models: Cell lines MDCK (NBL-2) cells ATCC Cat # CCL-34 Primary Bronchial/Tracheal epithelial cells; normal human ATCC Cat #PCS-300-010 Vero cells ATCC Cat #CCL-81 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Mouse Strain: Jax 000664; RRID: IMSR_JAX:000664 Mouse: B6.129S2-Ighmtm1Cgn/J Jackson Laboratory Mouse Strain: 002288; RRID: IMSR_JAX:002288 Mouse: C57BL/6N Germ free Taconic Bioscience Mouse Strain: C57BL/6NTac; RRID: IMSR_TAC:B6 Oligonucleotides KiCqStartTM Primer: Actb Forward: CATTGCTGACAGGATGCAGAAGG Millipore Sigma N/A KiCqStartTM Primer: Actb Reverse: TGCTGGAAGGTGGACAGTGAGG Millipore Sigma N/A KiCqStartTM Primer: Irf9 Forward: CTACTTCTGTAGAGATTTGGC Millipore Sigma N/A KiCqStartTM Primer: Irf9 Reverse: GATGAGATTCTCTTGGCTATG Millipore Sigma N/A (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e9, June 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStartTM Primer: Isg15 Forward: ATGGAGGACTTTTGGGATAG Millipore Sigma N/A KiCqStartTM Primer: Isg15 Reverse: AGAGGCAGAGCTTTTTATTG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Forward: CTACGCAAATCTGATCAAGG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Reverse: CACTTGTCTCTCACATACTG Millipore Sigma N/A KiCqStartTM Primer: Stat1 Forward: TTTGACAGTATGATGAGCAC Millipore Sigma N/A KiCqStartTM Primer: Stat1 Reverse: AGCAAATGTGATGCTCTTTC Millipore Sigma N/A Lactobacillus johnsonii 16S–23S region.

    RNA Sequencing:

    Article Title: Maternal helminths rewire the microbiota to promote offspring antiviral immunity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin, TPCK Treated Thermo Fisher Cat #PI20233 Trypsin-EDTA (0.25%) phenol red Thermo Fisher Cat #25200056 Vancomycin hydrochloride Millipore Sigma Cat #94747-5G Critical commercial assays ArCTM Amine Reactive Compensation Bead Kit Thermo Fisher Cat #A10346 BD Cytofix/Cytoperm BD Biosciences Cat #554722 BD Perm/Wash BD Biosciences Cat #554723 Brilliant Stain Buffer BD Bioscience Cat #566349 DNeasy 96 PowerSoil Pro Kit QIAGEN Cat #47017 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientifc (eBiocience) Cat #00-5523-00 Human IFN-beta Quantikine ELISA Kit Bio-Techne Cat #DIFNB0 IFN-beta Quantikine ELISA Kit Bio-Techne Cat #MIFNB0 iScriptTM cDNA Synthesis Kit Bio-Rad Cat #1708891 LEGENDplexTM Mouse Anti-Virus Response Panel (13-plex) BioLegend Cat #740622 LIVE/DEADTM Fixable Blue Dead Cell Stain Kit Thermo Fischer Cat #L34962 OneComp eBeadsTM Compensation Beads Thermo Fisher Cat #01-1111-42 QuantiNova SYBR Green PCR Kit QIAGEN Cat #208054 RNeasy Micro kit QIAGEN Cat #74004 RNeasy Mini kit QIAGEN Cat #73404 Quick-DNA Fecal/Soil Microbe Miniprep Kit Zymo Research Cat #D6010 Deposited data Raw RNA-seq data This paper GEO: GSE299495 Code and associated analysis This paper Figshare: https://doi.org/10.6084/ m9.figshare.31955649 Raw 16S rRNA-seq data This paper NCBI SRA: PRJNA1450632 Experimental models: Cell lines MDCK (NBL-2) cells ATCC Cat # CCL-34 Primary Bronchial/Tracheal epithelial cells; normal human ATCC Cat #PCS-300-010 Vero cells ATCC Cat #CCL-81 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory Mouse Strain: Jax 000664; RRID: IMSR_JAX:000664 Mouse: B6.129S2-Ighmtm1Cgn/J Jackson Laboratory Mouse Strain: 002288; RRID: IMSR_JAX:002288 Mouse: C57BL/6N Germ free Taconic Bioscience Mouse Strain: C57BL/6NTac; RRID: IMSR_TAC:B6 Oligonucleotides KiCqStartTM Primer: Actb Forward: CATTGCTGACAGGATGCAGAAGG Millipore Sigma N/A KiCqStartTM Primer: Actb Reverse: TGCTGGAAGGTGGACAGTGAGG Millipore Sigma N/A KiCqStartTM Primer: Irf9 Forward: CTACTTCTGTAGAGATTTGGC Millipore Sigma N/A KiCqStartTM Primer: Irf9 Reverse: GATGAGATTCTCTTGGCTATG Millipore Sigma N/A (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e9, June 10, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER KiCqStartTM Primer: Isg15 Forward: ATGGAGGACTTTTGGGATAG Millipore Sigma N/A KiCqStartTM Primer: Isg15 Reverse: AGAGGCAGAGCTTTTTATTG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Forward: CTACGCAAATCTGATCAAGG Millipore Sigma N/A KiCqStartTM Primer: Oasl1 Reverse: CACTTGTCTCTCACATACTG Millipore Sigma N/A KiCqStartTM Primer: Stat1 Forward: TTTGACAGTATGATGAGCAC Millipore Sigma N/A KiCqStartTM Primer: Stat1 Reverse: AGCAAATGTGATGCTCTTTC Millipore Sigma N/A Lactobacillus johnsonii 16S–23S region.

    Cell Culture:

    Article Title: Dictamnus dasycarpus Turcz. attenuates airway inflammation and mucus hypersecretion by modulating the STAT6-STAT3/FOXA2 pathway.
    Article Snippet: Inflammatory cells in the bronchoalveolar lavage fluid (BALF) were cytospinned onto slides, fixed, and stained with Diff-Quik (38721; Sysmex Co., Kobe, Japan) according to the manufacturer’s protocols. .. Primary bronchial and tracheal epithelial cells (HBECs; PCS300–010, ATCC, Manassas, VA, USA) at passage 2 were cultured in an airway epithelial cell basal medium (ATCC, PCS-300–030) supplemented with a bronchial epithelial cell growth kit (ATCC, PCS300–040). ..

    Article Title: Bone morphogenetic protein signaling regulation of AMPK and PI3K in lung cancer cells and C. elegans
    Article Snippet: A549 and H1299 cell lines obtained from patients with non-small cell lung cancer (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma Aldrich, Saint Louis, MO, USA) with 5% fetal bovine serum. .. Normal Human Primary Bronchial/Tracheal Epithelial Cells were from ATCC (PCS-300-010) and cultured in Airway Epithelial Cell Basal Medium (PCS-300-030) supplemented with Bronchial Epithelial Cell Growth Kit (PCS-300-040). .. JL5 was synthesized by John Gilleran and Jacques Roberge from Rutgers Molecular Design and Synthesis, and DMH1 (S7146) was purchased from Selleckchem.

    Article Title: Oncostatin-M ligand-based CAR-T therapy displays robust anti-tumor activity against osteosarcoma.
    Article Snippet: Jeko-1 cells (Jeko-1, CRL-3006) were purchased directly from ATCC and cultured in RPMI-1640 (SH30027.01, Cytiva) with 10% FBS and 1% Pen/Strep. .. Primary human bronchial/ tracheal epithelial cells (PCS-300-010) were purchased from ATCC and cultured in Airway Epithelial Cell Basal Medium (PCS-300-030, ATCC) with Bronchial Epithelial Cell Growth Kit (PCS-300-040, ATCC) per ATCC instructions. .. Primary human hepatocytes (5200, Sciencell) were purchased from Sciencell Research Laboratories and cultured in hepatocyte medium (5201) with 5mL of Hepatocyte Growth Supplement (5252, Sciencell) as well as 5% FBS, and 1% Pen/Strep.

    Modification:

    Article Title: Dexamethasone eluting polydopaminated polycaprolactone-poly (lactic-co-glycolic) acid for treatment of tracheal stenosis.
    Article Snippet: Advanced Platform Center, Louis Stokes Cleveland Veteran Affairs Medical Center, Cleveland, Ohio, USA Department of Urology, Case Western Reserve University, Cleveland, Ohio, USA Department of Mechanical and Aerospace Engineering, Case Western Reserve University, Cleveland, Ohio, USA Department of Biomedical Engineering, Case Western Reserve University, Cleveland, Ohio, USA Department of Orthopedic Surgery, University Hospitals Cleveland Medical Center, Cleveland, Ohio, USA Department of Otolaryngology, Case Western Reserve University, Cleveland, Ohio, USA

    Expressing:

    Article Title: Exosomes decorated with a recombinant SARS-CoV-2 receptor-binding domain as an inhalable COVID-19 vaccine.
    Article Snippet: SARS-N: https://www.novusbio.com/products/sars-nucleocapsid-protein-antibody_nb100-56576 CD3: https://www.abcam.com/cd3-antibody-sp7-ab16669.html MPO: https://www.thermofisher.com/antibody/product/Myeloperoxidase-Antibody-Polyclonal/PA5-16672 MX1: https://www.emdmillipore.com/US/en/product/Anti-MxA-clone-M143-CL143,MM_NF-MABF938 goat anti-rabbit HRP secondary antibody: https://www.abcam.com/goat-rabbit-igg-hl-hrp-ab6721.html goat anti-mouse HRP secondary antibody: https://www.abcam.com/goat-mouse-igg-hl-hrp-ab6789.html AF-488-CD206: https://www.scbt.com/p/cd206-antibody-d-1?requestFrom=search Alexa Fluor®568-Iba-1: https://www.abcam.com/alexa-fluor-568-iba1-antibody-epr61362-ab221003.html goat anti-rabbit Alexa Fluor® 647: https://www.abcam.com/goat-rabbit-igg-hl-alexa-fluor-647-ab150079.html FITC-pan-CK: https://www.abcam.com/pan-cytokeratin-antibody-c-11-fitc-ab78478.html Eukaryotic cell lines Policy information about cell lines Cell line source(s) Lung spheroid cells were generated from healthy human whole lung samples from the Cystic Fibrosis and Pulmonary Diseases Research and Treatment Center at the University of North Carolina at Chapel Hill. .. Primary small airway epithelial cells (PCS-301-010) and primary bronchial/tracheal epithelial cells (PCS-300-010) were purchased from ATCC (Manassas, VA, USA) A549 lung carcinoma cells expressing human ACE2 and TMPRSS2 (a549-hace2tpsa) were purchased from Invivogen (San Diego, CA, USA) C57BL/6 Dendritic cells (1129-4807AU20) were purchased from Cellero (Lowell, MA, USA) Authentication Primary small airway epithelial cells and primary bronchial/tracheal epithelial cells were authenticated by ATCC. .. A549 cells were authenticated by Invivogen and C57BL/6 dendritic cells were authenticated by Cellero, respectively.



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    ATCC human normal bronchial epithelial cells
    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
    Human Normal Bronchial Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
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    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
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    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
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    ATCC bronchial tracheal epithelial cells pbec
    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
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    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
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    ATCC epithelial cells primary lung atcc pcs 300 010
    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial <t>epithelial</t> cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial <t>(HBE)</t> cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
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    Image Search Results


    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial epithelial cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial (HBE) cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.

    Journal: iScience

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    doi: 10.1016/j.isci.2026.117627

    Figure Lengend Snippet: SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial epithelial cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial (HBE) cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.

    Article Snippet: Human bronchial epithelial cell line HBE , American Type Culture Collection , N/A.

    Techniques: Co-Immunoprecipitation Assay, Western Blot, Transfection, Immunofluorescence, Staining, Homologous Recombination, Reporter Assay, Flow Cytometry, Analysis, Knockdown, Apoptosis Assay

    Journal: iScience

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    doi: 10.1016/j.isci.2026.117627

    Figure Lengend Snippet:

    Article Snippet: Human bronchial epithelial cell line HBE , American Type Culture Collection , N/A.

    Techniques: Cell-Signaling, Recombinant, Transfection, Caspase-3 Activity Assay, Protein Extraction, Knock-Out, Sequencing, Control, Plasmid Preparation, Software, Analysis